Our Nanostring nCounter MAX System analyzes hundreds of mRNAs, miRNAs, SNVs, CNVs or proteins through direct digital molecular detection, in a single reaction step and in the absence of enzymes (without the need for retrotranscription or amplification steps). This highly sensitive and reproducible system, with enhanced multiplexing capacity (up to 800 genes in the same reaction), not only reduces the number of reactions required, but also the amount of RNA/DNA sample required for the assay.
The Illumina NextSeq2000 system features sequencing by synthesis (SBS) technology that tracks the addition of labeled nucleotides as the DNA chain is copied. This technology analyzes millions of fragments in massively parallel sequencing, improving the speed and accuracy of sequencing while reducing its cost.
The Illumina NextSeq 2000 Sequencing System features a novel super resolution optics system that yields highly accurate imaging data with greater resolution and higher sensitivity than traditional Illumina systems.
NGSChromium X Single Cell Platform available in our NGSservice
Advantages of the 10x Genomics technology:
This technology provides 3' gene expression to profile 3,000 to 10,000 cells per sample, identify different cell types, explore cellular heterogeneity and compare samples before and after treatment.
This solution analyzes T-cell and B-cell receptors, cell surface protein expression, antigen specificity and/or CRISPR edits, along with gene expression, all from a single cell.
It allows users to profile the regulatory landscape of chromatin in thousands of cells.
Multiomic profiling of the transcriptome and chromatin accessibility in the same cell enhances the characterization of cell types and states, and provides deeper insights into underlying gene regulatory mechanisms. Targeted gene expression to analyze the expression of a defined set of transcripts that are relevant to a specific research area of interest using single-cell resolution.
We provide omics data processing and debugging. We advise on pipeline assembly (standard and ad hoc) and custom software development.
Processing of the entire DNA analysispipeline, from FastQ to variant annotation. Quality control at any point in theprocess.
Population structure analysis of samplegroups, identification of phenotype-associated markers and genes, functionalanalysis of pathways and data mining (search and processing) of databases inpublic repositories for alternative analyses.
Expression analysis on RNA-Seq and Nanostringplatforms. Quality control, alignment and expression quantification at the geneor transcript level, and annotation.
Normalization and differential expression analysis and functional analysis of pathways.
Data analysis using the Illumina Infinium Methylation 450K and EPIC BeadChip platforms: quality control, normalization and detection of differentially methylated positions (DMPs) and regions (DMRs)in different sample groups. Analysis of gene pathways associated with DMPs andDRMs.